Weighted Geometric Mean (WGM) method: A new chromatic adaptation model.

The human visual system has undergone evolutionary changes to develop sophisticated mechanisms that enable stable color perception under varying illumination. These mechanisms are known as chromatic adaptation, a fundamental aspect of color vision. Chromatic adaptation can be divided into two categories: sensory adaptation, which involves automatic adjustments in the visual system, such as retinal gain control, in response to changes in the stimulus, and cognitive adaptation, which depends on the observer's knowledge of the scene and context. The geometric mean has been suggested to be the fundamental mathematical relationship that governs peripheral sensory adaptation. This paper proposes the WGM model, an advanced chromatic adaptation model based on a weighted geometric mean approach that can anticipate incomplete adaptation as it moves along the Planckian or Daylight locus. Compared with two other chromatic adaptation models (CAT16 and vK20), the WGM model is tested with different corresponding color data sets and found to be a significantly improvement while also predicting degree of adaptation (sensory and cognitive adaptation) in a physiologically plausible manner.

Protection of photosystem I during sudden light stress depends on ferredoxin:NADP(H) reductase abundance and interactions.

Plant tolerance to high light and oxidative stress is increased by overexpression of the photosynthetic enzyme Ferredoxin:NADP(H) reductase (FNR), but the specific mechanism of FNR-mediated protection remains enigmatic. It has also been reported that the localization of this enzyme within the chloroplast is related to its role in stress tolerance. Here, we dissected the impact of FNR content and location on photoinactivation of photosystem I (PSI) and photosystem II (PSII) during high light stress of Arabidopsis (Arabidopsis thaliana). The reaction center of PSII is efficiently turned over during light stress, while damage to PSI takes much longer to repair. Our results indicate a PSI sepcific effect, where efficient oxidation of the PSI primary donor (P700) upon transition from darkness to light, depends on FNR recruitment to the thylakoid membrane tether proteins: thylakoid rhodanase-like protein (TROL) and translocon at the inner envelope of chloroplasts 62 (Tic62). When these interactions were disrupted, PSI photoinactivation occurred. In contrast, there was a moderate delay in the onset of PSII damage. Based on measurements of ΔpH formation and cyclic electron flow, we propose that FNR location influences the speed at which photosynthetic control is induced, resulting in specific impact on PSI damage. Membrane tethering of FNR therefore plays a role in alleviating high light stress, by regulating electron distribution during short-term responses to light.

Impaired Light Adaptation of ON-Sustained Ganglion Cells in Early Diabetes Is Attributable to Diminished Response to Dopamine D4 Receptor Activation.

Purpose: Retinal neuronal signaling is disrupted early in diabetes, before the onset of the vascular pathologies associated with diabetic retinopathy. There is also growing evidence that retinal dopamine, a neuromodulator that mediates light adaptation, is reduced in early diabetes. Previously, we have shown that after 6 weeks of diabetes, light adaptation is impaired in ON-sustained (ON-s) ganglion cells in the mouse retina. The purpose of this study was to determine whether changes in the response to dopamine receptor activation contribute to this dysfunction. Methods: Single-cell retinal patch-clamp recordings from the mouse retina were used to determine how activating dopamine type D4 receptors (D4Rs) changes the light-evoked and spontaneous excitatory inputs to ON-s ganglion cells, in both control and 6-week diabetic (STZ-injected) animals. Fluorescence in situ hybridization was also used to assess whether D4R expression was affected by diabetes. Results: D4R activation decreased light-evoked and spontaneous inputs to ON-s ganglion cells in control and diabetic retinas. However, D4R activation caused a smaller reduction in light-evoked excitatory inputs to ON-s ganglion cells in diabetic retinas compared to controls. This impaired D4R signaling is not attributable to a decline in D4R expression, as there was no change in D4R mRNA density in the diabetic retinas. Conclusions: These results suggest that the cellular response to dopamine signaling is disrupted in early diabetes and may be amenable to chronic dopamine supplementation therapy.

Insights into Gene Regulation of Jasmonate-Induced Whole-Plant Senescence of Tobacco under Non-Starvation Conditions.

Jasmonate (JA)-induced plant senescence has been mainly studied with a dark/starvation-promoted system using detached leaves; yet, the induction of whole-plant senescence by JA remains largely unclear. This work reports the finding of a JA-induced whole-plant senescence of tobacco under light/non-starvation conditions and the investigation of underlying regulations. Methyl jasmonate (MeJA) treatment induces the whole-plant senescence of tobacco in a light-intensity-dependent manner, which is suppressed by silencing of NtCOI1 that encodes the receptor protein of JA-Ile (the bioactive derivative of JA). MeJA treatment could induce the senescence-specific cysteine protease gene SAG12 and another cysteine protease gene SAG-L1 to high expression levels in the detached leaf patches under dark conditions but failed to induce their expression in tobacco whole plants under light conditions. Furthermore, MeJA attenuates the RuBisCo activase (RCA) level in the detached leaves but has no effect on this protein in the whole plant under light conditions. A genome-wide transcriptional assay also supports the presence of a differential regulatory pattern of senescence-related genes during MeJA-induced whole-plant senescence under non-starvation conditions and results in the finding of a chlorophylase activity increase in this process. We also observed that the MeJA-induced senescence of tobacco whole plants is reversible, which is accompanied by a structural change of chloroplasts. This work provides novel insights into JA-induced plant senescence under non-starvation conditions and is helpful to dissect the JA-synchronized process of whole-plant senescence.

Toward predicting photosynthetic efficiency and biomass gain in crop genotypes over a field season.

Photosynthesis acclimates quickly to the fluctuating environment in order to optimize the absorption of sunlight energy, specifically the photosynthetic photon fluence rate (PPFR), to fuel plant growth. The conversion efficiency of intercepted PPFR to photochemical energy (ɛe) and to biomass (ɛc) are critical parameters to describe plant productivity over time. However, they mask the link of instantaneous photochemical energy uptake under specific conditions, that is, the operating efficiency of photosystem II (Fq'/Fm'), and biomass accumulation. Therefore, the identification of energy- and thus resource-efficient genotypes under changing environmental conditions is impeded. We long-term monitored Fq'/Fm' at the canopy level for 21 soybean (Glycine max (L.) Merr.) and maize (Zea mays) genotypes under greenhouse and field conditions using automated chlorophyll fluorescence and spectral scans. Fq'/Fm' derived under incident sunlight during the entire growing season was modeled based on genotypic interactions with different environmental variables. This allowed us to cumulate the photochemical energy uptake and thus estimate ɛe noninvasively. ɛe ranged from 48% to 62%, depending on the genotype, and up to 9% of photochemical energy was transduced into biomass in the most efficient C4 maize genotype. Most strikingly, ɛe correlated with shoot biomass in seven independent experiments under varying conditions with up to r = 0.68. Thus, we estimated biomass production by integrating photosynthetic response to environmental stresses over the growing season and identified energy-efficient genotypes. This has great potential to improve crop growth models and to estimate the productivity of breeding lines or whole ecosystems at any time point using autonomous measuring systems.

High-Light-Induced Stress Activates Lipid Deacylation at the Sn-2 Position in the Cyanobacterium Synechocystis Sp. PCC 6803.

Cyanobacterial mutants defective in acyl-acyl carrier protein synthetase (Aas) produce free fatty acids (FFAs) because the FFAs generated by deacylation of membrane lipids cannot be recycled. An engineered Aas-deficient mutant of Synechocystis sp. PCC 6803 grew normally under low-light (LL) conditions (50 µmol photons m-2 s-1) but was unable to sustain growth under high-light (HL) conditions (400 µmol photons m-2 s-1), revealing a crucial role of Aas in survival under the HL conditions. Several-times larger amounts of FFAs were produced by HL-exposed cultures than LL-grown cultures. Palmitic acid accounted for ∼85% of total FFAs in HL-exposed cultures, while C18 fatty acids (FAs) constituted ∼80% of the FFAs in LL-grown cultures. Since C16 FAs are esterified to the sn-2 position of lipids in the Synechocystis species, it was deduced that HL irradiation activated deacylation of lipids at the sn-2 position. Heterologous expression of FarB, the FFA exporter protein of Neisseria lactamica, prevented intracellular FFA accumulation and rescued the growth defect of the mutant under HL, indicating that intracellular FFA was the cause of growth inhibition. FarB expression also decreased the 'per-cell' yield of FFA under HL by 90% and decreased the proportion of palmitic acid to ∼15% of total FFA. These results indicated that the HL-induced lipid deacylation is triggered not by strong light per se but by HL-induced damage to the cells. It was deduced that there is a positive feedback loop between HL-induced damage and lipid deacylation, which is lethal unless FFA accumulation is prevented by Aas.

Maintaining the Chloroplast Redox Balance through the PGR5-Dependent Pathway and the Trx System Is Required for Light-Dependent Activation of Photosynthetic Reactions.

Light-dependent activation of chloroplast enzymes is required for the rapid induction of photosynthesis after a shift from dark to light. The thioredoxin (Trx) system plays a central role in this process. In chloroplasts, the Trx system consists of two pathways: the ferredoxin (Fd)/Trx pathway and the nicotinamide adenine dinucleotide phosphate (NADPH)-Trx reductase C (NTRC) pathway. In Arabidopsis (Arabidopsis thaliana) mutants defective in either pathway, the photoreduction of thiol enzymes was impaired, resulting in decreased carbon fixation. The close relationship between the Fd/Trx pathway and proton gradient regulation 5 (PGR5)-dependent photosystem I cyclic electron transport (PSI CET) in the induction of photosynthesis was recently elucidated. However, how the PGR5-dependent pathway is involved in the NTRC pathway is unclear, although NTRC has been suggested to physically interact with PGR5. In this study, we analyzed Arabidopsis mutants lacking either the PGR5 or the chloroplast NADH dehydrogenase-like complex (NDH)-dependent PSI CET pathway in the ntrc mutant background. The ntrc pgr5 double mutant suppressed both the growth defects and the high non-photochemical quenching phenotype of the ntrc mutant when grown under long-day conditions. By contrast, the inactivation of NDH activity with the chlororespiratory reduction 2-2 mutant failed to suppress either phenotype. We discovered that the phenotypic rescue of ntrc by pgr5 is caused by the partial restoration of Trx-dependent reduction of thiol enzymes. These results suggest that electron partitioning to the PGR5-dependent pathway and the Trx system needs to be properly regulated for the activation of the Calvin-Benson-Bassham cycle enzymes during the induction of photosynthesis.

Response analysis of fluorescence parameters of tomato seedlings oriented to vertical light environment adaptation.

Seedling quality greatly affects the subsequent survival, quality and yield of tomatoes. To explore the response of tomato seedlings on vertical light, we investigated the continuous trends of chlorophyll fluorescence parameters in six vertical light intensities and Pearson's correlation analysis of them. The results showed that the dark fluorescence parameters of Fm, Fv/Fm highly correlated with the photosynthetic photon flux density (PPFD) while NPQ, Y(NPQ), Y(NO) were highly correlated with the day of light processing (DLP). With increasing PPFD, the Fv/Fm decreased, the residual sum of curves increased and the scaling factor (S) was decreased. The photoinhibition phenomenon was relieved to different degrees on DLP 4. L4 (243.17 ± 4.37 µmol m-2 s-1) was the fastest light adaptation, L5 (295.34 ± 5.42 µmol m-2 s-1) was the second. ΦPSII accumulation was greatest in L4 and second in L5. Both L4 and L5 seedling health index and dry weight were significantly higher than L1 (53.20 ± 1.55 µmol m-2 s-1). L4 had the highest Chl a/b and total soluble sugar. It can be concluded that L4 was the best vertical PPFD with the highest light-adaption. The larger the PPFD, the greater the curve deviation, the greater the degree of data discretization, and the higher the photoinhibition. The more appropriate the light intensity is, the faster the seedlings light-adapted are. Therefore, the rapid and proper adjustment of light intensity is the key to obtain high quality tomato seedlings.

Dopamine D1 and D4 receptors contribute to light adaptation in ON-sustained retinal ganglion cells.

The adaptation of ganglion cells to increasing light levels is a crucial property of the retina. The retina must respond to light intensities that vary by 10-12 orders of magnitude, but the dynamic range of ganglion cell responses covers only ∼3 orders of magnitude. Dopamine is a crucial neuromodulator for light adaptation and activates receptors in the D1 and D2 families. Dopamine type D1 receptors (D1Rs) are expressed on horizontal cells and some bipolar, amacrine, and ganglion cells. In the D2 family, D2Rs are expressed on dopaminergic amacrine cells and D4Rs are primarily expressed on photoreceptors. However, the roles of activating these receptors to modulate the synaptic properties of the inputs to ganglion cells are not yet clear. Here, we used single-cell retinal patch-clamp recordings from the mouse retina to determine how activating D1Rs and D4Rs changed the light-evoked and spontaneous excitatory inputs to ON-sustained (ON-s) ganglion cells. We found that both D1R and D4R activation decrease the light-evoked excitatory inputs to ON-s ganglion cells, but that only the sum of the peak response decrease due to activating the two receptors was similar to the effect of light adaptation to a rod-saturating background. The largest effects on spontaneous excitatory activity of both D1R and D4R agonists was on the frequency of events, suggesting that both D1Rs and D4Rs are acting upstream of the ganglion cells.NEW & NOTEWORTHY Dopamine by bright light conditions allows retinal neurons to reduce sensitivity to adapt to bright light conditions. It is not clear how and why dopamine receptors modulate retinal ganglion cell signaling. We found that both D1 and D4 dopamine receptors in photoreceptors and inner retinal neurons contribute significantly to the reduction in sensitivity of ganglion cells with light adaptation. However, light adaptation also requires dopamine-independent mechanisms that could reflect inherent sensitivity changes in photoreceptors.

PIF4: Integrator of light and temperature cues in plant growth.

Plants are sessile and lack behavioural responses to avoid extreme environmental changes linked to annual seasons. For survival, they have evolved elaborate sensory systems coordinating their architecture and physiology with fluctuating diurnal and seasonal temperatures. PHYTOCHROME-INTERACTING FACTOR 4 (PIF4) was initially identified as a key component of the Arabidopsis thaliana phytochrome signalling pathway. It was then identified as playing a central role in promoting plant hypocotyl growth via the activation of auxin synthesis and signalling-related genes. Recent studies expanded its known regulatory functions to thermomorphogenesis and defined PIF4 as a central molecular hub for the integration of environmental light and temperature cues. The present review comprehensively summarizes recent progress in our understanding of PIF4 function in Arabidopsis thaliana, including PIF4-mediated photomorphogenesis and thermomorphogenesis, and the contribution of PIF4 to plant growth via the integration of environmental light and temperature cues. Remaining questions and possible directions for future research on PIF4 are also discussed.

Roles of alternative electron flows in response to excess light in Ginkgo biloba.

Ginkgo biloba L., the only surviving species of Ginkgoopsida, is a famous relict gymnosperm, it may provide new insight into the evolution of photosynthetic mechanisms. Flavodiiron proteins (FDPs) are conserved in nonflowering plants, but the role of FDPs in gymnosperms has not yet been clarified. In particular, how gymnosperms integrate FDPs and cyclic electron transport (CET) to better adapt to excess light is poorly understood. To elucidate these questions, we measured the P700 signal, chlorophyll fluorescence and electrochromic shift signal under fluctuating and constant light in G. biloba. Within the first seconds after light increased, G. biloba could not build up a sufficient proton gradient (ΔpH). Concomitantly, photo-reduction of O2 mediated by FDPs contributed to the rapid oxidation of P700 and protected PSI under fluctuating light. Therefore, in G. biloba, FDPs mainly protect PSI under fluctuating light at acceptor side. Under constant high light, the oxidation of PSI and the induction of non-photochemical quenching were attributed to the increase in ΔpH formation, which was mainly caused by the increase in CET rather than linear electron transport. Therefore, under constant light, CET finely regulates the PSI redox state and non-photochemical quenching through ΔpH formation, protecting PSI and PSII against excess light. We conclude that, in G. biloba, FDPs are particularly important under fluctuating light while CET is essential under constant high light. The coordination of FDPs and CET fine-tune photosynthetic apparatus under excess light.

Pre-illumination at high light significantly alleviates the over-reduction of photosystem I under fluctuating light.

Photosystem I (PSI) is the primary target of photoinhibition under fluctuating light (FL). In angiosperms, cyclic electron flow (CEF) around PSI is thought to be the main player protecting PSI under FL. The activation of CEF is linked to the thylakoid stacking, which is in turn affected by light intensity. However, it is unknown how pre-illumination affects the CEF activation and PSI redox state under FL. To address this question, we conducted a spectroscopic analysis under FL in Bletilla striata and Morus alba after pre-illumination at moderate light (ML, 611 µmol photons m-2 s-1) or high light (HL, 1455 µmol photons m-2 s-1). Our results indicated that both species displayed a transient over-reduction of PSI after a transition from low to high light, but the extent of PSI over-reduction under FL was largely alleviated by pre-illumination at HL when compared with pre-illumination under ML. Furthermore, pre-illumination at HL accelerated the activation rate of CEF but did not affect the activation of non-photochemical quenching and linear electron flow from photosystem II under FL. Therefore, such increased CEF activity by pre-illumination under HL alleviated PSI over-reduction under FL by facilitating the electron sink downstream of PSI. Taking together, pre-illumination at HL protects PSI under FL through acceptor-side regulation.

Analyses of the photosynthetic characteristics, chloroplast ultrastructure, and transcriptome of apple (Malus domestica) grown under red and blue lights.

BACKGROUND: Light quality significantly affects plant growth and development, photosynthesis, and carbon and nitrogen metabolism. Apple (Malus domestica Borkh.) is a widely cultivated and economically important fruit crop worldwide. However, there are still few studies on the effects of different light qualities on the growth and development of apple seedlings. RESULTS: In this study, we explored the effects of blue and red light treatments on the growth and development, photosynthetic characteristics, leaf chloroplast ultrastructure, and carbon and nitrogen metabolism of apple seedlings. Blue light significantly inhibited apple plant growth and leaf extension, but it promoted the development of leaf tissue structures and chloroplasts and positively affected leaf stomatal conductance, the transpiration rate, and photosynthetic efficiency. The red light treatment promoted apple plant growth and root development, but it resulted in loosely organized leaf palisade tissues and low chlorophyll contents. The blue and red light treatments enhanced the accumulation of ammonium nitrogen in apple seedlings. Moreover, the blue light treatment significantly promoted nitrogen metabolism. Additionally, an RNA-seq analysis revealed that both blue light and red light can significantly up-regulate the expression of genes related to carbon and nitrogen metabolism. Blue light can also promote amino acid synthesis and flavonoid metabolism, whereas red light can induce plant hormone signal transduction. The expression of a gene encoding a bHLH transcription factor (MYC2-like) was significantly up-regulated in response to blue light, implying it may be important for blue light-mediated plant development. CONCLUSIONS: Considered together, blue and red light have important effects on apple growth, carbon and nitrogen metabolism. These findings may be useful for determining the ideal light conditions for apple cultivation to maximize fruit yield and quality.

Photosynthetic regulation in response to fluctuating light conditions under temperature stress in three mosses with different light requirements.

Under natural field conditions, mosses experience fluctuating light intensities combined with temperature stress. Alternative electron flow mediated by flavodiiron proteins (FLVs) and cyclic electron flow (CEF) around photosystem I (PSI) allow mosses to growth under fluctuating light conditions. However, little is known about the roles of FLVs and CEF in the regulation of photosynthesis under temperature stress combined with fluctuating light. Here, we measured chlorophyll fluorescence and P700 redox state under fluctuating light conditions at 4 °C, 20 °C, and 35 °C in three mosses with different light requirements. Upon a sudden increase in light intensity, electron flow from photosystem II initially increased and then gradually decreased at 20 °C and 35 °C, indicating that the operation of FLV-dependent flow lasted much longer than previously thought. Furthermore, the absolute rates of FLV-dependent flow and CEF were enhanced under fluctuating light at 35 °C, pointing to their important roles in photoprotection when exposed to fluctuating light at moderate high temperature. Furthermore, the downregulation of FLV activity at 4 °C was partially compensated for by enhanced CEF activity. These results suggested the subtle coordination between FLV activity and CEF under fluctuating light and temperature stress. Racomitrium japonicum and Hypnum plumaeforme, which usually grow under relatively high light levels, exhibited higher FLV activity and CEF than the shade-grown moss Plagiomnium ellipticum. Based on our results, we conclude that photosynthetic acclimation to fluctuating light and temperature stress in different mosses is largely linked to the adjustment of FLV activity and CEF.

Effects of light, anoxia and salinity on the expression of dihydroxyacid dehydratase in maize.

Dihydroxyacid dehydratase (EC 4.2.1.9) participates in metabolism of branched chain amino acids, in CoA biosynthesis and in the conversion of hydroxycitric acid that accumulates in several plants. In maize (Zea mays L.), this enzyme is encoded by the two genes (Dhad1 and Dhad2), having different patterns of their expression during germination. We have demonstrated the inhibition of Dhad1 expression by light and the opposite effect of light on Dhad2. These effects were phytochrome-dependent and involved methylation/demethylation of promoters. Incubation of maize plants in a nitrogen atmosphere resulted in Dhad1 activation peaking at 12 h, which coincided with the decrease in promoter methylation. The gene Dhad2 was activated only during the first 6 h of anoxia, with no correlation with the level of promoter methylation. Salt stress (150 mM NaCl) caused the activation of expression of Dhad2 while the expression of Dhad1 was inhibited in the first hour and then after 12 h incubation with NaCl. We conclude that the expression of two genes encoding dihydroxyacid dehydratase reveals the opposite or different patterns of regulation by light, anoxia and salinity. The mechanisms underlying these modifications involve promoter methylation and result in corresponding changes in the enzymatic activity of the conversion of hydroxycitrate to 2-oxoglutarate.

Exogenous carbon source supplementation counteracts root and hypocotyl growth limitations under increased cotyledon shading, with glucose and sucrose differentially modulating growth curves.

Plant growth is continuously modulated by endogenous and exogenous stimuli. By no means the only, but well described, signaling molecules produced in plants and distributed through the plant body to orchestrate efficient growth are photosynthates. Light is a potent exogenous stimulus that determines, first, the rate of photosynthesis, but also the rate of plant growth. Root meristem activity is reduced with direct illumination but enhanced with increased sugar levels. With reduced cotyledon illumination, the seedling increases hypocotyl elongation until adequate light exposure is again provided. If endogenous carbon sources are limited, this leads to a temporary inhibition of root growth. Experimental growth conditions include exogenous supplementation of sucrose or glucose in addition to culturing seedlings under light exposure in Petri dishes. We compared total root length and hypocotyl elongation of Arabidopsis thaliana wild type Col-0 in response to illumination status and carbon source in the growth medium. Overall, sucrose supplementation promoted hypocotyl and root length to a greater extent than glucose supplementation. Glucose promoted root length compared to non-supplemented seedlings especially when cotyledon illumination was greatly reduced.

A theoretical framework of the hybrid mechanism of photosystem II photodamage.

Photodamage of photosystem II is a significant physiological process that is prevalent in the fields of photobiology, photosynthesis research and plant/algal stress. Since its discovery, numerous efforts have been devoted to determine the causes and mechanisms of action of photosystem II photodamage. There are two contrasting hypotheses to explain photodamage: (1) the excitation pressure induced by light absorption by the photosynthetic pigments and (2) direct photodamage of the Mn cluster located at the water-splitting site, which is independent of excitation pressure. While these two hypotheses seemed mutually exclusive, during the last decade, several independent works have proposed an alternative approach indicating that both hypotheses are valid. This was termed the dual hypothesis of photosystem II photodamage, and it postulates that both excess excitation and direct Mn photodamage operate at the same time, independently or in a synergic manner, depending on the type of sample, temperature, light spectrum, or other environmental stressors. In this mini-review, a brief summary of the contrasting hypotheses is presented, followed by recapitulation of key discoveries in the field of photosystem II photodamage of the last decade, and a synthesis of how these works support a full hybrid framework (operation of several mechanisms and their permutations) to explain PSII photodamage. All these are in recognition of Prof. Wah Soon Chow (the Australian National University), one of the key proposers of the dual hypothesis.

Cryo-EM photosystem I structure reveals adaptation mechanisms to extreme high light in Chlorella ohadii.

Photosynthesis in deserts is challenging since it requires fast adaptation to rapid night-to-day changes, that is, from dawn's low light (LL) to extreme high light (HL) intensities during the daytime. To understand these adaptation mechanisms, we purified photosystem I (PSI) from Chlorella ohadii, a green alga that was isolated from a desert soil crust, and identified the essential functional and structural changes that enable the photosystem to perform photosynthesis under extreme high light conditions. The cryo-electron microscopy structures of PSI from cells grown under low light (PSILL) and high light (PSIHL), obtained at 2.70 and 2.71 Å, respectively, show that part of light-harvesting antenna complex I (LHCI) and the core complex subunit (PsaO) are eliminated from PSIHL to minimize the photodamage. An additional change is in the pigment composition and their number in LHCIHL; about 50% of chlorophyll b is replaced by chlorophyll a. This leads to higher electron transfer rates in PSIHL and might enable C. ohadii PSI to act as a natural photosynthesiser in photobiocatalytic systems. PSIHL or PSILL were attached to an electrode and their induced photocurrent was determined. To obtain photocurrents comparable with PSIHL, 25 times the amount of PSILL was required, demonstrating the high efficiency of PSIHL. Hence, we suggest that C. ohadii PSIHL is an ideal candidate for the design of desert artificial photobiocatalytic systems.